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https://colab.research.google.com/drive/1QceecEr7DwBsp6A8hnvufPpFbT1CAB2u
https://documenter.getpostman.com/view/21901492/UzXSuuWY
https://ello.co/gramormtiofu/post/dxptwfuudnuptxe_1bbhtg
https://colab.research.google.com/drive/1Oq-O39I9A4nTpZGQIKFj_8-baZGVURrm
https://ello.co/procacfdest_no/post/etg2iiri3fb1fq3wr_-zew
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https://ello.co/clerunalpuz/post/27yykoqqtum-s3zyvi_cta
https://colab.research.google.com/drive/1VEIBDrBq3cQbu3Iz5J5eHs0AsUbnYfyp
https://documenter.getpostman.com/view/21851018/UzXVrt1a
The general objective of this research is to identify some of the proteins in polynucleated cells of mammals which have important functions in nuclear organization. The physical isolation of these proteins and their chemical characterization will be undertaken. Particular attention will be given to the nuclear matrix which appears to be an amorphous complex containing the large number of proteins which are associated with the nuclear matrix. We plan to use the metrizamide density gradient centrifugation technique to determine the physical characteristics of the nuclear matrix and to begin to characterize its major proteins. The localization of these proteins within the nuclear matrix will be determined by applying fluorescent antibody techniques and electron microscopy. Hopefully this will answer the question of the function of the nuclear matrix and identify some of the proteins which are implicated in nuclear activity. In addition, we will examine the organization of the nuclear matrix in various cells at various stages of the cell cycle. This will involve the colocalization of several biochemical markers in order to identify the stage of the cell cycle at which the matrix is assembled and organized. Finally, an attempt will be made to devise a new method for the visualization of intranuclear protein distribution in three-dimensional space. This will be accomplished by the use of a fluorescent derivative of alpha-cyanoacetamide which will colocalize with proteins stained by immunofluorescence techniques. This technique will be applied to the mammalian cell.This project is designed to build understanding of the structure of motor control and how that might be influenced in Parkinson’s disease. The fundamental hypothesis of the work is that there are two common features of parkinsonian speech that reflect dysfunction of the motor speech system: impairments in the speed of articulation and altered kinematics of the speech process. It is further hypothesized that these elements are independent of each other, and therefore their disruptions should be additive, contributing to impairments in quality of life for people with Parkinson’s disease. A series of natural-speech experiments will test the two hypotheses. In response to a speech signal, people with Parkinson’s disease will produce various sequences of movement, and then motor speech performance will be evaluated. People with Parkinson’s disease are expected to show impaired movements in response to the speech signal. In addition, because impairments in speech motor control are hypothesized to reflect disordered motor control, it is expected that people with Parkinson’s disease will have difficulty producing words at normal speech rates. The experiments are divided into three phases. Phase I is a pilot experiment which will introduce a natural-speech paradigm, examine the validity and reliability of
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