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Tissue-specific expression of bovine GH in liver and muscle cells of transgenic mice.
The development of a new transgenic mouse line was undertaken in order to study tissue-specific expression of bovine growth hormone (bGH) in vitro and in vivo. For this purpose, a construct containing the bGH cDNA sequence was used, and the cDNA fragment was microinjected into fertilized eggs of CD-1 mice. The transgenic mice were screened for the presence of the bGH cDNA by polymerase chain reaction and verified for the presence of bGH by means of immunohistochemistry. The transgenic mice exhibited a high, specific growth response in vivo. The highest levels of bGH were found in the serum, liver, testes, and in muscle tissues of 2-month-old transgenic mice, while in the brain no bGH could be detected. In transgenic mice that were about 1 yr old, the highest growth response was found in the liver and testes, but only low levels were seen in the brain. The age-related increase in the serum levels of bGH correlated to a decrease in the bGH levels in the brain of 2-month-old transgenic mice. The transgene expression was also tested in vitro using a bGH-responsive cell line, the L929-bGH cells. When this cell line was transfected with the bGH construct, the serum levels of bGH could be significantly increased by the presence of the transgene. The L929-bGH cells used in this study also retained the ligand
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