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The fate of benzophenones, one of the most frequently used UV-filter substances in sunscreens, was studied in human subjects. For this purpose, semisolid lotions, serums, and gels containing benzophenone-3 (BP-3) and -4 (BP-4) were applied on the forearm skin of human volunteers and the dermal and systemic disposition was determined. BP-3 was metabolized to 4-hydroxybenzophenone (4OH-BP-3). BP-4, on the other hand, was not found in the urine of any volunteers, even after application of BP-4 in gel (50 mg/cm2) or lotion (1 mg/cm2) formulations. Thus, BP-4 can be regarded as a very persistent UV-filter. The amount of BP-3 excreted unchanged in urine was small (max. 0.4 microg/mg creatinine) and unchanged BP-3 was not detected in the urine of volunteers. BP-3, as well as its 4OH-metabolite, was extensively accumulated in the skin after topical application. However, only small amounts of BP-4 accumulated in the skin, possibly because of its low penetration potential. BP-4 was also metabolized to 4OH-BP-4. The amount of BP-3 and 4OH-BP-3 recovered in serum after application of BP-3 gel was greater than that of BP-3 applied in the lotion or serum formulation. This indicates that serum levels of BP-3, and especially of 4OH-BP-

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He’s the clown prince of all time. He may not even be a prince. But at the box office, Joker is king. The DC Comics-inspired origin story has become the highest-grossing film in Warner Bros. movie history, catapulting Joaquin Phoenix to superstar status.

“This is the highest-grossing (studio) film of all time,” said Warner Bros. domestic distribution chief Jeff Goldstein.

“Joker” was released Thursday, two days before Thanksgiving weekend, and it has grossed a stellar $161 million. It is the second-highest-grossing R-rated movie of all time (behind only “Deadpool”), the fastest film ever to cross $500 million and now it’s knocking “Star Wars: The Force Awakens” out of the top spot.

The film, which carries a $135 million price tag, has racked up $375 million domestically and $676 million worldwide, making it the highest-grossing original R-rated movie ever. “A Star is Born,” which was released earlier this year, was a few million less. It cost $35 million.

It is also the biggest weekend ever for Warner Bros. this Thanksgiving weekend, beating the record of $40.2 million a year ago when “Batman v

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